Composite

Part:BBa_K2235006:Design

Designed by: Shivashree Dhanaraj, Shanlin Tong, Gilai Nachmann and Sina Amoor pour   Group: iGEM17_Stockholm   (2017-10-11)


Sialidase enzyme with a RBS attached


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 73
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 520
    Illegal NgoMIV site found at 595
    Illegal NgoMIV site found at 685
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1065


Design Notes

HIS-tag at the N-terminal


Source

BBa_B0034 (RBS from iGEM Antiquity) BBa_K2235005 (Sialidase enzyme made by iGEM Stockholm 2017)

References